What is the reference range for Pregnenolone (by LC-MS/MS)?

The standard reference range for Pregnenolone (by LC-MS/MS) is Adults: approximately 10–230 ng/dL (0.3–7.3 nmol/L), strongly age-, sex-, and (in women) cycle-dependent. Reference ranges vary by laboratory and assay platform — interpret in context of the issuing laboratory's stated range.

Which tests measure Pregnenolone (by LC-MS/MS)?

Pregnenolone (by LC-MS/MS) is measured in 1 test on BiomarkersLabs.com, including Pregnenolone (LC-MS/MS) Test.

Biomarker Reference · PREG

What does the Pregnenolone (by LC-MS/MS) test measure?

Pregnenolone (by LC-MS/MS) (PREG) is a clinical biomarker used in adrenal-stress assessment. Pregnenolone is the first product of steroidogenesis, formed from cholesterol by the side-chain cleavage enzyme (CYP11A1) in mitochondria — the rate-limiting committed step of steroid synthesis. It is measured in 1 laboratory test on BiomarkersLabs.com.

Reference Range: Adults: approximately 10–230 ng/dL (0.3–7.3 nmol/L), strongly age-, sex-, and (in women) cycle-dependent
Units: ng/dL, nmol/L
Measured In: 1 test
Category: Adrenal

Functional medicine optimalMid-range values are sometimes cited as favourable in functional medicine, though no standardised optimal applies

What is Pregnenolone (by LC-MS/MS)?

Pregnenolone is the first product of steroidogenesis, formed from cholesterol by the side-chain cleavage enzyme (CYP11A1) in mitochondria — the rate-limiting committed step of steroid synthesis. It is the common upstream precursor for all steroid hormones, feeding the progestogen, glucocorticoid, mineralocorticoid, androgen, and oestrogen pathways, and is produced in the adrenal cortex, gonads, and brain (as a neurosteroid). It circulates at low concentrations with a short half-life.

Why is Pregnenolone (by LC-MS/MS) measured?

Pregnenolone is measured mainly to characterise steroidogenesis — most usefully to help localise enzymatic blocks in congenital adrenal hyperplasia and related steroidogenic disorders, and as an upstream reference point when downstream steroids are abnormal. Measured by LC-MS/MS, it avoids the cross-reactivity that makes immunoassay pregnenolone unreliable. It is also used in some functional and integrative 'adrenal precursor' assessments, an application whose interpretation is not well established.

Reference ranges

Standard reference: Adults: approximately 10–230 ng/dL (0.3–7.3 nmol/L), strongly age-, sex-, and (in women) cycle-dependent
Functional medicine optimal: Mid-range values are sometimes cited as favourable in functional medicine, though no standardised optimal applies
Units: ng/dL, nmol/L

Reference ranges vary by laboratory and assay platform. Interpret in context of the issuing laboratory's stated range.

Tests that measure Pregnenolone (by LC-MS/MS)

1 test on BiomarkersLabs.com include this biomarker.

BML-ADR-014Comprehensive

Pregnenolone (LC-MS/MS) Test

Pregnenolone (by LC-MS/MS) reference range in this test: Adults: approximately 10–230 ng/dL (0.3–7.3 nmol/L), strongly age-, sex-, and (in women) cycle-dependent

Related clinical conditions

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