Which tests measure Free Testosterone (calculated)?

Free Testosterone (calculated) is measured in 1 test on BiomarkersLabs.com, including Blood Spot LCMS Hormones 5 with SHBG.

Biomarker Reference · Free T

What does the Free Testosterone (calculated) test measure?

Q: What is the reference range for Free Testosterone (calculated)?

The standard reference range for Free Testosterone (calculated) is Calculated from total testosterone, SHBG, and albumin (assumed constant); sex- and age-specific, typically 50–200 pg/mL in adult males, 1–10 pg/mL in females. Method- and algorithm-dependent.. Reference ranges vary by laboratory and assay platform — interpret in context of the issuing laboratory's stated range.

Free Testosterone (calculated) (Free T) is a clinical biomarker used in male-hormones assessment. Free testosterone represents the unbound, biologically active fraction, approximately 1–3 per cent of total testosterone. It is measured in 1 laboratory test on BiomarkersLabs.com.

Reference Range: Calculated from total testosterone, SHBG, and albumin (assumed constant); sex- and age-specific, typically 50–200 pg/mL in adult males, 1–10 pg/mL in females. Method- and algorithm-dependent.
Units: pg/mL
Measured In: 1 test
Category: Male Hormones

Functional medicine optimalFunctional practitioners often target mid-to-upper reference-range free testosterone for symptom relief, but clinical thresholds are contested and individualised.

What is Free Testosterone (calculated)?

Free testosterone represents the unbound, biologically active fraction, approximately 1–3 per cent of total testosterone. Because direct free-testosterone immunoassays are unreliable, free testosterone is calculated from total testosterone and SHBG using empirical equations (Vermeulen or similar) that estimate the partition between bound and free hormone.

Why is Free Testosterone (calculated) measured?

Calculated free testosterone refines the assessment of androgen status when SHBG is abnormal — high SHBG can mask bioavailable androgen deficiency, low SHBG can mask hyperandrogenism. The calculation improves diagnostic sensitivity for hypogonadism and polycystic ovary syndrome, but its accuracy depends on the total testosterone and SHBG assays and the equation used.

Reference ranges

Standard reference: Calculated from total testosterone, SHBG, and albumin (assumed constant); sex- and age-specific, typically 50–200 pg/mL in adult males, 1–10 pg/mL in females. Method- and algorithm-dependent.
Functional medicine optimal: Functional practitioners often target mid-to-upper reference-range free testosterone for symptom relief, but clinical thresholds are contested and individualised.
Units: pg/mL

Reference ranges vary by laboratory and assay platform. Interpret in context of the issuing laboratory's stated range.

Tests that measure Free Testosterone (calculated)

1 test on BiomarkersLabs.com include this biomarker.

BML-HRM-015Comprehensive

Blood Spot LCMS Hormones 5 with SHBG

Free Testosterone (calculated) reference range in this test: Calculated from total testosterone, SHBG, and albumin (assumed constant); sex- and age-specific, typically 50–200 pg/mL in adult males, 1–10 pg/mL in females. Method- and algorithm-dependent.

Related clinical conditions

Related Biomarkers

Frequently measured alongside

Testosterone (LC-MS dried blood spot)T1 test Estradiol (LC-MS dried blood spot)E21 test Progesterone (LC-MS dried blood spot)P41 test Dehydroepiandrosterone Sulphate (LC-MS dried blood spot)DHEA-S1 test Cortisol (LC-MS dried blood spot)F1 test Sex Hormone Binding Globulin (dried blood spot)SHBG1 test

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