What is the reference range for 16α-Hydroxyestrone (dried urine, GCMS)?

The standard reference range for 16α-Hydroxyestrone (dried urine, GCMS) is Laboratory-specific; interpreted relative to 2-OHE1.. Reference ranges vary by laboratory and assay platform — interpret in context of the issuing laboratory's stated range.

Which tests measure 16α-Hydroxyestrone (dried urine, GCMS)?

16α-Hydroxyestrone (dried urine, GCMS) is measured in 1 test on BiomarkersLabs.com, including Blood Spot Thyroids + Dried Urine GCMS Estrogen Hormone Metabolites.

Biomarker Reference · 16α-OHE1

What does the 16α-Hydroxyestrone (dried urine, GCMS) test measure?

16α-Hydroxyestrone (dried urine, GCMS) (16α-OHE1) is a clinical biomarker used in thyroid-endocrine assessment. 16α-Hydroxyestrone is formed by CYP3A4-mediated hydroxylation at C-16. It is measured in 1 laboratory test on BiomarkersLabs.com.

Reference Range: Laboratory-specific; interpreted relative to 2-OHE1.
Units: ng/mg creatinine
Measured In: 1 test
Category: Thyroid

Functional medicine optimalLower 16α-OHE1 relative to 2-OHE1 (high 2:16α ratio) is the functional-medicine target, though this remains investigational.

What is 16α-Hydroxyestrone (dried urine, GCMS)?

16α-Hydroxyestrone is formed by CYP3A4-mediated hydroxylation at C-16. It is a potent oestrogen that binds covalently to the oestrogen receptor, creating a long-lived receptor–ligand complex that drives sustained transcriptional activity and cellular proliferation.

Why is 16α-Hydroxyestrone (dried urine, GCMS) measured?

16α-OHE1 has been studied as a biomarker of proliferative oestrogenic activity and potential breast cancer risk. A low 2:16α ratio is often flagged in functional practice as indicating 'unfavourable' metabolism, though epidemiological studies have yielded mixed results and ratio-based interventions lack definitive outcome trials.

Reference ranges

Standard reference: Laboratory-specific; interpreted relative to 2-OHE1.
Functional medicine optimal: Lower 16α-OHE1 relative to 2-OHE1 (high 2:16α ratio) is the functional-medicine target, though this remains investigational.
Units: ng/mg creatinine

Reference ranges vary by laboratory and assay platform. Interpret in context of the issuing laboratory's stated range.

Tests that measure 16α-Hydroxyestrone (dried urine, GCMS)

1 test on BiomarkersLabs.com include this biomarker.

BML-THY-032Comprehensive

Blood Spot Thyroids + Dried Urine GCMS Estrogen Hormone Metabolites

16α-Hydroxyestrone (dried urine, GCMS) reference range in this test: Laboratory-specific; interpreted relative to 2-OHE1.

Related clinical conditions

Related Biomarkers

Frequently measured alongside

Thyroid-Stimulating HormoneTSH5 tests Free ThyroxineFT45 tests Free TriiodothyronineFT35 tests Thyroid Peroxidase Antibodies (dried blood spot)TPOAb3 tests Estrone (dried urine, GCMS)E11 test Estradiol (dried urine, GCMS)E21 test Estriol (dried urine, GCMS)E31 test 2-Hydroxyestrone (dried urine, GCMS)2-OHE11 test

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