Which tests measure 25-Hydroxyvitamin D, Total (D2 + D3)?

25-Hydroxyvitamin D, Total (D2 + D3) is measured in 1 test on BiomarkersLabs.com, including Functional Health Panel (Female).

Biomarker Reference · 25-OH Vit D

What does the 25-Hydroxyvitamin D, Total (D2 + D3) test measure?

Q: What is the reference range for 25-Hydroxyvitamin D, Total (D2 + D3)?

The standard reference range for 25-Hydroxyvitamin D, Total (D2 + D3) is Deficient: 100 ng/mL # REVIEWER FLAG: Vitamin D sufficiency threshold is contested — Endocrine Society defines sufficiency as >30 ng/mL, Institute of Medicine as >20 ng/mL. Confirm Access Medical Laboratories threshold and flag the guideline discordance inline.. Reference ranges vary by laboratory and assay platform — interpret in context of the issuing laboratory's stated range.

25-Hydroxyvitamin D, Total (D2 + D3) (25-OH Vit D) is a clinical biomarker used in comprehensive-panels assessment. 25-Hydroxyvitamin D is the major circulating form and the accepted clinical marker of vitamin D status, reflecting both cutaneous synthesis from solar UVB exposure and dietary/supplemental intake. It is measured in 1 laboratory test on BiomarkersLabs.com.

Reference Range: Deficient: <20 ng/mL (<50 nmol/L); Insufficient: 20–29 ng/mL; Sufficient: 30–100 ng/mL; Toxic: >100 ng/mL # REVIEWER FLAG: Vitamin D sufficiency threshold is contested — Endocrine Society defines sufficiency as >30 ng/mL, Institute of Medicine as >20 ng/mL. Confirm Access Medical Laboratories threshold and flag the guideline discordance inline.
Units: ng/mL, nmol/L
Measured In: 1 test
Category: —

Functional medicine optimal40–70 ng/mL (functional medicine optimal, targeting the mid-to-upper sufficient range for immune and non-skeletal benefits beyond fracture prevention). # REVIEWER FLAG: Functional optimal of 40–70 ng/mL is widely cited in integrative practice but exceeds the Institute of Medicine sufficiency definition — reviewer to confirm framing and which guideline framework to reference.

What is 25-Hydroxyvitamin D, Total (D2 + D3)?

25-Hydroxyvitamin D is the major circulating form and the accepted clinical marker of vitamin D status, reflecting both cutaneous synthesis from solar UVB exposure and dietary/supplemental intake. It is produced by hepatic 25-hydroxylation of vitamin D3 (cholecalciferol) and D2 (ergocalciferol) and has a serum half-life of approximately 2–3 weeks — making it a reliable indicator of medium-term vitamin D status rather than acute intake.

Why is 25-Hydroxyvitamin D, Total (D2 + D3) measured?

Vitamin D deficiency is prevalent across female populations, particularly in those with limited sun exposure, darker skin phototypes, obesity, malabsorption, or exclusively indoor lifestyles. In female health panels, adequate vitamin D is relevant to bone mineral density maintenance, immune regulation, cardiovascular risk, mood and cognitive function, breast and colorectal cancer risk reduction, and fertility outcomes. Measurement is essential before supplementation dosing and for monitoring repletion protocol efficacy.

Reference ranges

Standard reference: Deficient: <20 ng/mL (<50 nmol/L); Insufficient: 20–29 ng/mL; Sufficient: 30–100 ng/mL; Toxic: >100 ng/mL # REVIEWER FLAG: Vitamin D sufficiency threshold is contested — Endocrine Society defines sufficiency as >30 ng/mL, Institute of Medicine as >20 ng/mL. Confirm Access Medical Laboratories threshold and flag the guideline discordance inline.
Functional medicine optimal: 40–70 ng/mL (functional medicine optimal, targeting the mid-to-upper sufficient range for immune and non-skeletal benefits beyond fracture prevention). # REVIEWER FLAG: Functional optimal of 40–70 ng/mL is widely cited in integrative practice but exceeds the Institute of Medicine sufficiency definition — reviewer to confirm framing and which guideline framework to reference.
Units: ng/mL, nmol/L

Reference ranges vary by laboratory and assay platform. Interpret in context of the issuing laboratory's stated range.

Tests that measure 25-Hydroxyvitamin D, Total (D2 + D3)

1 test on BiomarkersLabs.com include this biomarker.

BML-CHP-001Comprehensive

Functional Health Panel (Female)

25-Hydroxyvitamin D, Total (D2 + D3) reference range in this test: Deficient: <20 ng/mL (<50 nmol/L); Insufficient: 20–29 ng/mL; Sufficient: 30–100 ng/mL; Toxic: >100 ng/mL # REVIEWER FLAG: Vitamin D sufficiency threshold is contested — Endocrine Society defines sufficiency as >30 ng/mL, Institute of Medicine as >20 ng/mL. Confirm Access Medical Laboratories threshold and flag the guideline discordance inline.

Related clinical conditions

Related Biomarkers

Frequently measured alongside

Complete Blood Count with DifferentialCBC w/ Diff1 test Comprehensive Metabolic PanelCMP1 test Thyroid-Stimulating HormoneTSH9 tests Free ThyroxineFree T49 tests Free TriiodothyronineFree T38 tests EstradiolE22 tests Progesterone4 tests Follicle-Stimulating HormoneFSH3 tests

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