What is the reference range for 16α-Hydroxyestrone?

The standard reference range for 16α-Hydroxyestrone is Premenopausal 3–12 ng/mg Cr; postmenopausal 2–8 ng/mg Cr. Method- and laboratory-specific.. Reference ranges vary by laboratory and assay platform — interpret in context of the issuing laboratory's stated range.

Biomarker Reference · 16α-OHE1

What does the 16α-Hydroxyestrone test measure?

Q: Which tests measure 16α-Hydroxyestrone?

16α-Hydroxyestrone is measured in 3 tests on BiomarkersLabs.com, including Blood Spot Thyroids + Dried Urine GCMS Estrogen Hormone Metabolites and LCMS Diurnal Hormones I, Dried Urine GCMS Estrogen Hormone Metabolites and LCMS Diurnal Hormones I, Essential Estrogens™ - FMV.

16α-Hydroxyestrone (16α-OHE1) is a clinical biomarker used in female-hormones assessment. 16α-Hydroxyestrone is produced by CYP3A4-mediated 16α-hydroxylation of estrone. It is measured in 3 laboratory tests on BiomarkersLabs.com.

Reference Range: Premenopausal 3–12 ng/mg Cr; postmenopausal 2–8 ng/mg Cr. Method- and laboratory-specific.
Units: pg/mg creatinine, ng/mg creatinine
Measured In: 3 tests
Category: Female Hormones

Functional medicine optimalFunctional sources propose lower 16α-OHE1 relative to 2-OHE1; however, this remains investigational and should be presented as part of a personalised risk discussion rather than a diagnostic endpoint.

What is 16α-Hydroxyestrone?

16α-Hydroxyestrone is produced by CYP3A4-mediated 16α-hydroxylation of estrone. It retains significant oestrogenic activity and can form covalent adducts with the oestrogen receptor, resulting in prolonged receptor occupancy. Observational studies have linked higher 16α-OHE1 or a lower 2:16α ratio to greater proliferative signalling in breast tissue.

Why is 16α-Hydroxyestrone measured?

Practitioners measure 16α-hydroxyestrone to characterise the balance of oestrogen metabolism. A high 16α-OHE1 or low 2-OHE1:16α-OHE1 ratio is considered by some to reflect increased proliferative exposure, though evidence is observational and lifestyle or pharmacological interventions targeting this ratio lack robust clinical trial support.

Reference ranges

Standard reference: Premenopausal 3–12 ng/mg Cr; postmenopausal 2–8 ng/mg Cr. Method- and laboratory-specific.
Functional medicine optimal: Functional sources propose lower 16α-OHE1 relative to 2-OHE1; however, this remains investigational and should be presented as part of a personalised risk discussion rather than a diagnostic endpoint.
Units: pg/mg creatinine, ng/mg creatinine

Reference ranges vary by laboratory and assay platform. Interpret in context of the issuing laboratory's stated range.

Tests that measure 16α-Hydroxyestrone

3 tests on BiomarkersLabs.com include this biomarker.

BML-THY-031Comprehensive

Blood Spot Thyroids + Dried Urine GCMS Estrogen Hormone Metabolites and LCMS Diurnal Hormones I

16α-Hydroxyestrone reference range in this test: Dried urine: proprietary method-specific intervals; no consensus clinical reference.

BML-HRF-023Comprehensive

Dried Urine GCMS Estrogen Hormone Metabolites and LCMS Diurnal Hormones I

16α-Hydroxyestrone reference range in this test: Typically 0.5–4 ng/mg creatinine.

BML-HRF-026Comprehensive

Essential Estrogens™ - FMV

16α-Hydroxyestrone reference range in this test: Premenopausal 3–12 ng/mg Cr; postmenopausal 2–8 ng/mg Cr. Method- and laboratory-specific.

Related clinical conditions

Related Biomarkers

Frequently measured alongside

EstroneE15 tests EstradiolE25 tests EstriolE35 tests 2-Hydroxyestrone2-OHE14 tests 4-Hydroxyestrone4-OHE14 tests 2-Methoxyestrone2-MeOE14 tests 4-Methoxyestrone4-MeOE13 tests 2-Hydroxyestradiol2-OHE22 tests

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